Description
Description: This assay employs a two-site sandwich ELISA to quantitate YAP1 in samples. An antibody specific for YAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: YAP1 contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.
The human and mouse cDNAs encode deduced 493- and 531-amino acid proteins, respectively, with significant sequence similarity to the chicken protein. All 3 contain a putative protein module of 38 amino acids that is found in various structural, regulatory, and signaling molecules in yeast, nematode, and mammals and may be involved in protein-protein interaction. The mouse protein contains an imperfect repeat of the module. Because one of the prominent features of the sequence motif is 2 tryptophans, it has been designated the WW domain